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Phospho-Antibody array results showing the regulatory effects of ZXF on HF. (n=6). ( A ) The overview of phospho-antibody array results. ( B ) The figure of PDK1 (Phospho-Ser241), PDK1 (Ab-241), FOXO1/3/4-pan (Phospho-Thr24/32) and FOXO1/3/4-pan (Ab-24/32). ( C and D ) Statistical results of p-PDK1/PDK1, and p-FOXO1/3/4/FOXO1/3/4. * P <0.05, ** P <0.01 vs Con group; Δ P <0.05 vs Dox group.

Journal: Drug Design, Development and Therapy

Article Title: Regulatory Effects of Zhenxin Formula in Treating Doxorubicin-Induced Heart Failure: Network Pharmacology and Animal Experimental Verification

doi: 10.2147/DDDT.S513643

Figure Lengend Snippet: Phospho-Antibody array results showing the regulatory effects of ZXF on HF. (n=6). ( A ) The overview of phospho-antibody array results. ( B ) The figure of PDK1 (Phospho-Ser241), PDK1 (Ab-241), FOXO1/3/4-pan (Phospho-Thr24/32) and FOXO1/3/4-pan (Ab-24/32). ( C and D ) Statistical results of p-PDK1/PDK1, and p-FOXO1/3/4/FOXO1/3/4. * P <0.05, ** P <0.01 vs Con group; Δ P <0.05 vs Dox group.

Article Snippet: Anti-Caspase-3 antibody (D3R6Y), anti-AKT1 phospho (Thr308) antibody (4056S), anti-AKT1 antibody (2920S), anti-FOXO1 phospho (Ser256) antibody (9461T), anti-FOXO1 antibody (2880T), anti-PDK1 phospho (Ser241) antibody (3438T), and anti-PDK1 antibody (5662S) were purchased from CST.

Techniques: Ab Array

ZXF regulated the PI3K/PDK1/AKT/FoxO1 signaling pathway. (n=5). ( A ) Western blot of phosphorylated and total protein levels of PI3K, PDK1, AKT, and FOXO1. ( B – E ) Statistical results of p-PI3K/PI3K, p-PDK1/PDK1, p-AKT/AKT, and p-FOXO1/FOXO1. ** P <0.01 vs Con group; Δ P <0.05, ΔΔ P <0.01 vs Dox group.

Journal: Drug Design, Development and Therapy

Article Title: Regulatory Effects of Zhenxin Formula in Treating Doxorubicin-Induced Heart Failure: Network Pharmacology and Animal Experimental Verification

doi: 10.2147/DDDT.S513643

Figure Lengend Snippet: ZXF regulated the PI3K/PDK1/AKT/FoxO1 signaling pathway. (n=5). ( A ) Western blot of phosphorylated and total protein levels of PI3K, PDK1, AKT, and FOXO1. ( B – E ) Statistical results of p-PI3K/PI3K, p-PDK1/PDK1, p-AKT/AKT, and p-FOXO1/FOXO1. ** P <0.01 vs Con group; Δ P <0.05, ΔΔ P <0.01 vs Dox group.

Article Snippet: Anti-Caspase-3 antibody (D3R6Y), anti-AKT1 phospho (Thr308) antibody (4056S), anti-AKT1 antibody (2920S), anti-FOXO1 phospho (Ser256) antibody (9461T), anti-FOXO1 antibody (2880T), anti-PDK1 phospho (Ser241) antibody (3438T), and anti-PDK1 antibody (5662S) were purchased from CST.

Techniques: Western Blot

Illustration of the PI3K/PDK1/AKT/FoxO1 Signaling Pathway in Inhibiting Cardiomyocyte Apoptosis and the Role of ZXF.

Journal: Drug Design, Development and Therapy

Article Title: Regulatory Effects of Zhenxin Formula in Treating Doxorubicin-Induced Heart Failure: Network Pharmacology and Animal Experimental Verification

doi: 10.2147/DDDT.S513643

Figure Lengend Snippet: Illustration of the PI3K/PDK1/AKT/FoxO1 Signaling Pathway in Inhibiting Cardiomyocyte Apoptosis and the Role of ZXF.

Article Snippet: Anti-Caspase-3 antibody (D3R6Y), anti-AKT1 phospho (Thr308) antibody (4056S), anti-AKT1 antibody (2920S), anti-FOXO1 phospho (Ser256) antibody (9461T), anti-FOXO1 antibody (2880T), anti-PDK1 phospho (Ser241) antibody (3438T), and anti-PDK1 antibody (5662S) were purchased from CST.

Techniques: